¡¾ABSTRACT¡¿Nanochitin whisker (NC) is a rod-like particle with cationic nature and high biological activities. Crown rot, caused by soil-borne fungal pathogens including Fusarium pseudograminearum (Fp) and F. graminearum (Fg), is one of the most damaging diseases in wheat. In order to explore the antifungal activity of NC against wheat diseases, the effects of nanochitin whisker suspension (NCs) on fungal growth and conidial production of Fp and Fg were studied in vitro. NCs and its mixture with chemical fungicide as a seeds coating agent for crown rot disease control were also investigated using Fp as a model microorganism in a pot test. The results showed that NCs had significant inhibitory effects on mycelial growth and conidial production of Fp and Fg at concentrations of 30 ppm and 300 ppm in the growth medium. Particularly, 300 ppm of NCs was capable of reducing conidial formation 89.25% and 82.28% for Fp and Fg respectively. When seeds were treated with mixture of NCs and tebuconazole, the disease control efficiencies increased to 79.30% and 90.02% for NCs at concentrations of 10 ppm and 30 ppm respectively. Greener and shorter seedlings were also observed in the pot experiment. This indicates that NC have strong antifungal activity against the soil-borne pathogens of wheat and reduce use of chemical fungicide in wheat plantation.