Ning Wang, Di Cao, Fangping Gong, Lixia Ku, Yanhui Chen, Wei Wang

Journal of Proteomics
DOI: 10.1016/j.jprot.2015.07.027

¡¾ABSTRACT¡¿ The midrib of leaves provides the primary support for the blade and is largely associated with leaf angle size. To elucidate the role of the midrib in leaf angle , the line Shen137 (larger leaf angle) and a near isogenic line (NIL, smaller leaf angle) were used in the present study. The results of the analysis showed that both the puncture forces and proximal collenchyma number of the midribs of the first and second leaves above the ear were higher in NIL than in Shen137. Comparative proteomic analysis was performed to reveal profile differences in the midribs of the 5th, 10th and 19th newly expanded leaves between Shen137 and NIL. Quantitative analysis of 24 identified midrib indicated that the maximum changes in abundance of 22 between Shen137 and NIL appeared at the 10th leaf stage, of which , , and were implicated in glycometabolism. Thus, glycometabolism might be associated with leaf angle and the physical and mechanical properties of the midribs. These results provide insight into the mechanism underlying leaf angle .